Abselion, a pioneering life sciences expertise firm centered on simplifying biomolecule quantification, has expanded its product providing, with the launch of the AAVX Whole Capsid Quantification Package and the AAV9 Whole Capsid Quantification Package. Each kits are designed to be used with its Amperia™ benchtop quantification platform and embody Thermo Fisher Scientific’s CaptureSelect™ affinity reagents. Combining these trusted reagents with Abselion’s constant assay format reduces the necessity for in-house optimization. These kits supply researchers working in adeno-associated virus (AAV) improvement and characterization a extra streamlined and accessible workflow to generate reproducible titer measurements throughout a broad vary of serotypes and course of situations.
Abselion’s Amperia benchtop platform is a simple-to-use resolution for fast and correct automated quantification of a variety of biomolecules, with out optics, fluidics, or specialist coaching. Every equipment is equipped in a ready-to-use format, together with sensor strips, assay plates, matched detection reagents, and assay buffers for the binding and detection steps. The expanded equipment vary enhances the utility of Amperia for scientists working throughout gene remedy improvement and course of workflows, providing a sensible, reliable method to complete capsid quantification from purified or advanced samples.
Abselion has entered right into a licensing settlement with Thermo Fisher Scientific to include CaptureSelect affinity reagents into its AAV quantification kits. CaptureSelect expertise relies on recombinant single-domain antibodies designed for top goal specificity, low cross-reactivity, and constant batch efficiency to make sure sturdy titer quantification. With the mixing of those reagents, the brand new kits advance Abselion’s AAV assay format, bringing improved efficiency and broader serotype applicability.
Each kits use a sandwich-style immunoassay format, by which biotin- and HRP-conjugated CaptureSelect antibodies are sequentially utilized to bind AAV capsids. The ensuing sign is detected electrochemically utilizing Amperia sensor strips, enabling correct quantitative detection throughout a variety of AAV pattern sorts and concentrations.
The AAVX Whole Capsid Quantification Package consists of CaptureSelect Anti-AAVX Biotin and HRP Conjugates, enabling broad serotype protection together with AAV1-8 and AAVrh10. The AAV9 Whole Capsid Quantification Package incorporates CaptureSelect Anti-AAV9 Biotin and HRP Conjugates for centered quantification of AAV9 particles.
Dependable quantification of complete AAV capsid focus is essential to sustaining consistency and supporting knowledgeable decision-making. The combination of Thermo Fisher Scientific’s CaptureSelect reagents strengthens the efficiency of Abselion’s AAV kits and displays our deal with making high-quality titer measurement less complicated and extra accessible. With these additions, Amperia affords researchers a ready-to-use resolution that mixes trusted reagent applied sciences with a streamlined assay format, enabling dependable quantification throughout a wider vary of serotypes and improvement workflows.”
Dr Ruizhi Wang, CEO and Founder, Abselion
Dr Kelly Flook, Sr. Supervisor, Product Administration, Pharma Analytics, Thermo Fisher Scientific, mentioned: “CaptureSelect affinity reagents are designed to ship excessive specificity and lot-to-lot consistency, making them properly suited to purposes similar to AAV capsid quantification. We’re happy that Abselion has integrated these reagents underneath licence to assist their assay kits for the Amperia platform.”
